tayebeh hatami; Seyed Kamal Kazemitabar; Ghaffar Kiani; Reza Esmaeilzadeh kenari
Volume 3, Issue 1 , November 2017, , Pages 23-29
Abstract
The genus pumpkin from the family Cucurbitaceae with oily seeds has 10 species, 5 of which are ofagricultural importance. Three species C. Pepo and C. Moschata, C. Maxima are more important. ...
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The genus pumpkin from the family Cucurbitaceae with oily seeds has 10 species, 5 of which are ofagricultural importance. Three species C. Pepo and C. Moschata, C. Maxima are more important. The presentstudy aimed to investigate genetic diversity based on ISSR markers in 30 genotypes of 3 populations ofpumpkin. For this purpose, 4 primers with simple repetitive sequences (microsatellite) were used. In this study,72 genetic locations were ranked, of which there were 72 polymorphic locations with a polygonal percentageof 100%. To assess the genetic similarity between the population, cluster analysis using Jaccard's similaritywas used with UPGMA method. The mean genetic distance of genotypes (by Jaccard's coefficient of similarity)was 0.77 and the mean of content (PIC) was 0.47. The ISSR11 primers had the highest PIC (0.50). With theregret to dendrograms obtained from cluster analysis revealed a high variation among genotypes. The highestgenetic distance was between genotypes R30, R11 and R14 and the lowest genetic distance between R24 andR27. The results of this study showed that ISSR markers can be effectively used to study the genetic variationof pumpkin. Among the primers used, the marker ISSR11 ((GA) 8 G) was the most suitable primer forsubsequent studies.
